AI-assisted learning in exercise physiology: a quasi-experimental study using PhysioExercise GPT
- Design
- Quasi-experimental study · randomized at student level · 64 participants
- Subject and task
- Other
Exercise Physiology course content over one term (bioenergetics, energy metabolism, substrate utilization, hormonal regulation, cardiovascular and respiratory responses, homeostasis, training adaptations). Intervention students were told to use PhysioExercise GPT about 20-30 minutes per session at least three times a week, using ten Bloom-graded starter prompts (explanations, problem-based scenarios, case analysis, formative self-assessment with feedback); control students received lectures and guided activities and were told not to use AI tools.
Exposure: Contradictory in source. Tool access is stated as 22 July to 27 October 2024 (about 14 weeks) after a 7-day familiarization period; the same paper describes the follow-up as about six months (one academic semester) and characterizes tool use “over a 26-week follow-up period”, with the planned dose (52 uses) computed from 26 weeks. Not resolvable from the source. - Population match
- Secondary students: Different · University students: Direct
- Attrition
- Not reported. Students who did not complete T0 and/or T1, withdrew, or failed minimum participation requirements were excluded, but the number recruited, allocated, excluded and analysed is not given; 64 (32/32) is both the allocated and the analysed figure, which implies either zero attrition or allocation after exclusions (per-protocol). No participant flow is reported.
- Study tier
- Study tier 4Single course, single instructor, 64 students, non-registered, with allocation described as both a lottery and feasibility-based, and labelled non-randomized by the authors. The comparison is not a like-for-like contrast: the AI arm got a prescribed extra 60-90+ minutes a week of structured practice with adaptive quizzes and feedback (Table 3 reports a mean of 114 messages per participant), and the instructor's role differed between arms (facilitator of technology use vs lectures and guided activities), despite the claim that the only difference was the tool; the two arms shared a classroom, so contamination and differential teaching cannot be separated. The outcome is a 10-item multiple-choice test written and validated by the same experts who built the tool's knowledge base, whose domains are stated to correspond to that knowledge base, and whose administration conditions (supervision, AI/internet access) and date are not reported. Baseline was taken after the AI group's 7-day familiarization and favours the AI group by about 0.5 SD, untested. Attrition and flow are not reported. The internal timeline does not hold together (14-week access window vs 26-week follow-up; 93 mean days with interactions vs 43.81 mean interactions per participant; 2025 documents in a knowledge base used in 2024; base model stated as GPT-4.5 in July-October 2024). The reported arithmetic is internally consistent (gain difference, t, CI, g and the ANCOVA table all recompute), so the numbers are not in themselves implausible, but the design and reporting problems leave very little confidence that the 1.41-point gain difference reflects learning caused by the tool. VERY_LOW.
- Assessment
- Version 1 · AI: two passes agreed · Oct 29, 2026
Study facts come from the paper. Population match and study tier are our judgments, made separately for the two populations this collection covers.
At one Brazilian university, 64 Physical Education students in an Exercise Physiology course were split 32/32 (the paper calls this both a lottery and a non-random, feasibility-based allocation) into a group that also used a custom ChatGPT-based tutor built by the authors and a group taught conventionally in the same classroom. On a 10-question multiple-choice test written by the tool's own developers, the AI group gained 3.38 points versus 1.97 (difference 1.41 points, 95% CI 0.54 to 2.27; g = 0.80). The paper does not say whether students could use the AI during the final test or exactly when it was given, the AI group got extra structured practice time, baseline favoured the AI group, and the reported timeline and model version are internally inconsistent, so the result is very weak evidence about independent learning.
What the study measured
Arms
| Arm | What it got |
|---|---|
| A · PhysioExercise GPT (structured use of a custom ChatGPT-based tutor plus course instruction) n = 32 | Intervention · teacher not reported PhysioExercise GPT (custom ChatGPT-based tool built by the author group; current version labelled GPT-5.6) · GPT-4.5 as stated by the authors for the 2024 intervention period (see limitations_observed on the date conflict) · Prompted pedagogy · chat interface · not reported · used 2024-07-22..2024-10-27 |
| B · Conventional teaching (control) n = 32 | Control · teacher led Same course, curriculum, teaching schedule, instructor, and evaluation criteria; traditional teaching strategies including lectures and guided activities. Told not to use AI-based tools (compliance not verifiable); no access to PhysioExercise GPT. No matched extra practice time or materials reported. |
Findings
A finding is one outcome contrast: two arms, one measure, one set of conditions. A paper is never "positive" or "negative" here; each finding carries its own direction and its own conditions. The badge that matters most is the first one: whether the AI was still available when the outcome was measured. Why that gates everything.
| Finding and conditions | Result |
|---|---|
| F1 · Post-intervention (T1) Exercise Physiology test: 10 author-developed multiple-choice items (five options, one correct), scored 0-10; parallel form of T0; analysed as gain T1 - T0 between groups (primary analysis) and by ANCOVA on T1 adjusting for T0 PhysioExercise GPT (structured use of a custom ChatGPT-based tutor plus course instruction) vs Conventional teaching (control)AI availability at assessment not reported — cannot carry a learning claimImmediate post-test · T1 undated; read as the end-of-course assessmentSame contentResearcher-developed testPrespecification unclear | Benefit Gain difference +1.41 points on the 0-10 scale (AI gain 3.38 ± 1.60, T0 3.88 ± 1.41 to T1 7.25 ± 1.19; control gain 1.97 ± 1.86, T0 3.09 ± 1.67 to T1 5.06 ± 1.50); Hedges' g = 0.80 (on gain scores). Raw T1 difference 2.19 points (reviewer arithmetic). ANCOVA on T1 adjusting for T0: group F(1, 61) = 34.73, p < 0.001, partial eta squared 0.363 (SS 59.34; error MS 1.71); adjusted means and adjusted difference not reported. 0.54 to 2.27 (95% CI, gain difference); ANCOVA CI not reported t(62) = 3.244, p = 0.002 (gain difference); ANCOVA p < 0.001 Ten-item, author-developed multiple-choice test (the paper calls it “standardized”). The items were written by the same three experts who selected the PhysioExercise GPT knowledge base, and the paper says the item domains correspond to the content of that knowledge base, so the test is aligned with the AI arm's practice material. Content validity rests on an I-CVI of 1.00 from those same three experts; no reliability, item analysis, or form-equivalence data are reported, and the instruments are in the supplement (not read). Scored dichotomously by two blinded evaluators. Administration conditions and AI access at test not reported. |
- Limitations
- Stated by the authors: quasi-experimental design without randomization; possible selection bias; no a priori sample-size calculation (power analysis was post hoc); instrument of only ten items; reduced measurement sensitivity; confounders not controlled: baseline performance, attendance/participation, extracurricular study time, digital literacy, external AI use, motivation, interaction between groups; shared classroom: complete separation not feasible, risk of contamination; control students told not to use AI tools, but compliance could not be controlled; gains cannot be attributed exclusively to PhysioExercise GPT; results are associations, not causal; outcome predominantly theoretical, which the authors say may underestimate effects on applied domains. Observed by review: allocation is self-contradictory: feasibility-based (2.1), lottery “simple randomization” (2.3), and “without randomization” (abstract, limitations) in the same paper; reported to STROBE; the outcome test was written and content-validated by the experts who built the tool's knowledge base, and its domains are stated to correspond to that knowledge base: alignment risk favouring the AI arm; the I-CVI of 1.00 comes from the same three developers, not independent judges; conditions of T0/T1 administration (proctored or not, in class or online, whether PhysioExercise GPT or other AI/internet was accessible) are not reported, so AI availability at assessment cannot be established; T1 date is not reported; tool access ended 27 October 2024 while the follow-up is described as about six months / 26 weeks, so the interval between end of exposure and T1 is unknown; T0 was administered after the 7-day familiarization with the tool, so the AI group's baseline may already include tool exposure; it was also 0.79 points higher, untested; time-on-task and instructor-role confounding: the AI arm had prescribed extra practice (20-30 min, at least 3x/week) and a different instructor role; the paper still claims the only difference was the tool; no participant flow, attrition or exclusion counts; exclusion of students who missed assessments or minimum participation implies a per-protocol sample; number of AI-arm students who met the 39-use adherence threshold is not reported (only a mean usage rate of 84.25%); dose figures inconsistent: instruction was at least three uses a week, but the planned dose assumes two a week over 26 weeks; the access window is about 14 weeks; Table 3's 93 mean days with interactions exceeds its 43.81 mean interactions per participant; “Mean usage time*” has an undefined asterisk and no unit; knowledge base described as 58 documents published 1990-2025 although the tool version tested was used July-October 2024; base model stated as GPT-4.5 during July-October 2024; to the reviewer's knowledge (not from the source) OpenAI first released GPT-4.5 in February 2025, so the stated model/date pairing cannot both be correct; abstract claims “enhancing the retention of complex concepts” and a “robust” tool, but there is no delayed or retention test and the discussion says causal inference cannot be established; ANCOVA adjusted means, adjusted difference and its CI are not reported, only F, p and partial eta squared; double-blind scoring with averaging of two evaluators is described for dichotomously scored multiple-choice items, which would not ordinarily need judgment; the procedure is unexplained; consent statements conflict: “All participants provided informed consent” vs written consent from “legal guardians/next of kin” for an adult sample (mean age 21.3); a digital-literacy (ICILS-type) assessment is reported in Results with no description in Methods of when or how it was given, for whole sample only, with an implausibly narrow SD (12.1) for a scale whose proficiency bands are 84-104 points wide; in-text citations of Yildiz/Nisanci (2026) appear swapped against the reference list; one named peer reviewer (Onur Seçgin Nişanci) is an author of the works discussed at length in the Discussion; single course, single instructor, single institution; findings are for a custom GPT built and evaluated by the same team. Integrity note (IN-025, carried into limitations per the owner's ruling): both passes independently found the allocation described three ways, a baseline taken after the AI arm's tool familiarization, an access window shorter than the stated duration, no participant flow, a guardians/next-of-kin consent statement for adults, and a retention claim in the abstract with no delayed measure. Pass B adds, from outside knowledge, that the stated base model (GPT-4.5) was first released after the stated intervention dates, so the base model or the dates are misreported. Neither pass found anything showing fabrication; the pattern is careless reporting plus an anachronism that makes the intervention description unreliable.
Who was studied
- Level
- university
- Years
- 5th-semester undergraduate Physical Education students
- Ages
- mean 21.3 years (SD 3.21); range not reported
- Country
- BR
- Setting
- Faculty of Physical Education (FAEF), State University of Rio Grande do Norte (UERN), Mossoro, Brazil; regular Exercise Physiology classes; one instructor and one shared classroom for both groups. 71% female.
- Prior knowledge
- T0 means 3.88 (SD 1.41) AI vs 3.09 (SD 1.67) control on the 0-10 test; the 0.79-point baseline gap (about 0.5 SD) was not tested or discussed. T0 was given after the 7-day tool familiarization period, so it is not a clean pre-exposure baseline for the AI group. Self-reported daily study time 15.2 (SD 4.6) minutes and a digital-literacy score (581.4, SD 12.1, described as ICILS Level 3) are reported for the whole sample only, not by group.
Methodological notes
Extracted from: https://doi.org/10.3389/fphys.2026.1894046 Access: full text (Frontiers, CC BY), read from data/fulltext-cache/bundles/ribeirodasilva-2026.txt (PDF text first, then XML full text); Figure 1 is an image and the Supplementary Material (the T0/T1 instruments) is not in the bundle, so item content is not coded Allocation: Records the allocation level only, not a claim that allocation was random: individual students were assigned to arms (32/32) within a single class cohort. Whether that assignment was random is contradicted within the paper (see design_note). Analysis was student-level (paired and independent t-tests; ANCOVA), which matches the allocation unit, but the shared classroom means outcomes are not independent across arms. IN-025 rulings: randomization_level student records the allocation unit only (every account the paper gives allocates individual students; it does not imply randomness, per the syromyatnikov-2026 precedent). F1 timing immediate_post is a reading of the paper's “post-intervention” label, not a reported date. The ANCOVA is the same contrast re-analysed with no adjusted estimate, so it rides F1's estimate and p-value fields rather than a second finding.
Funding and conflicts
- Funding
- CAPES (Coordination for the Improvement of Higher Education Personnel), Brazil
- Vendor funded
- No
- Vendor
- None identified
- Notes
- Authors declare no commercial or financial relationships. The tool under test was built by the author group, and the same physiology experts who selected its knowledge base wrote and content-validated the outcome test (developer-evaluator overlap; no commercial vendor). Generative AI was used to build the tool and to organize citations.
Funding is shown on every study and never used to score it.
The source, as retrieved
Abstract
This study evaluated the applicability and effectiveness of PhysioExercise GPT, an artificial intelligence tool based on the ChatGPT architecture, developed to support the teaching of exercise physiology to undergraduate physical education students. A longitudinal quasi-experimental study was conducted involving a total of 64 students. Thirty-two students used the AI tool through a structured educational approach that incorporated personalized support, immediate feedback, and learning activities based on Bloom’s Taxonomy, while a control group of 32 students received traditional instruction. The results showed that both groups improved over time; however, the AI-assisted group achieved significantly greater learning gains (p = 0.002). The intervention group demonstrated a mean improvement of 3.38 points compared with 1.97 points in the control group, corresponding to a large effect size (Hedges’ g = 0.80). Analysis of covariance (ANCOVA) confirmed that the superiority of the AI-assisted group remained significant after adjusting for baseline performance. The findings indicate that the structured use of PhysioExercise GPT functions as an effective digital tutor, promoting active learning and enhancing the retention of complex concepts. Although the study has limitations, including the absence of randomization, the results suggest that AI represents a robust complementary educational tool capable of improving academic performance in health education.
Where this record came from
| Source | Retrieved | Identifier |
|---|---|---|
| monthly-sweep | Oct 28, 2026 | doi:10.3389/fphys.2026.1894046 Screened in 2026-09-30 (sweep item 5); metadata verified 2026-10-28 against OpenAlex, Crossref, PubMed and the Frontiers landing page; full text retrieved (open, CC-BY). |